CCL-37 RK-13 兔肾胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背细胞系，ATCC 细景清楚,提供参考文献和优培养条件
| ATCC® Number: | CCL-37™ | Price: | $339.00 |
| Designations: | RK13 |
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| | Depositors: | BC Meyer |
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| | Biosafety Level: | 2 [Cells contain Bovine Viral Diarrhea Virus （BVDV） ] |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | adherent |
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| | Organism: | Oryctolagus cuniculus （rabbit） |
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| | Morphology: | epithelial
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| | Source: | Organ: kidney Disease: normal |
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| | Cellular Products: | keratin |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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| | Applications: | transfection host （Roche FuGENE® Transfection Reagents） |
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| | Age: | 5 weeks CCL-37 RK-13 兔肾细胞系 |
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| | Comments: | The cells are positive for keratin by immunoperoxidase staining.The cells are positive for bovine viral diarrhea virus （BVDV）. |
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| | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide （CO2）, 5% Temperature: 37.0°C |
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| | Subculturing: | Protocol:Remove and discard culture medium. Briefly rinse the cell layer with 0.25% （w/v） Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: Twice per week |
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| | Preservation: | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2003 recommended serum:ATCC 30-世彩堂 0.25% （w/v） Trypsin - 0.53 mM EDTA in Hank' BSS （w/o Ca++, Mg++）:ATCC 30-2101 Cell culture tested DMSO:ATCC 4-X |
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| | References: | 18385: Didier ES, et al. Characterization of Encephalitozoon （Septata） intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708 22145: . . Lancet 2: 640, 1963. 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 26192: . . Lancet 2: 593, 1963. 28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 |
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